文献类型：期刊文献

英文题名：Yolk-shell magnetic mesoporous TiO2 microspheres with flowerlike NiO nanosheets for highly selective enrichment of phosphopeptides

作者：Hong, Yayun[1];Pu, Chenlu[1];Zhao, Hongli[1];Sheng, Qianying[1];Zhan, Qiliang[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：9

期号：43

起止页码：16764

外文期刊名：NANOSCALE

收录：;EI(收录号：20174604411982);WOS:【SCI-EXPANDED(收录号:WOS:000414960900029)】；

基金：This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 16520710800), the Fundamental Research Funds for the Central Universities (No. 222201717022), the Natural Science Foundation of Shanghai (No. 16ZR1407600) and the Shanghai Sailing Program (No. 16YF1402400).

语种：英文

外文关键词：Microspheres - Magnetite - Titanium dioxide - Lanthanum compounds - Shells (structures) - Fluorine compounds - Nanosheets

摘要：In this work, we fabricated a yolk-shell magnetic composite that contains mesoporous TiO2 as the inner shell and flowerlike NiO as the outer shell (denoted as Fe3O4@H-TiO2@f-NiO) to reduce the limitations of single-component metal oxides in phosphopeptide enrichment. The NiO nanosheets play a synergistic role in phosphopeptide enrichment. And the unique flowerlike structure of NiO with sufficient space can facilitate the reversible insertion/extraction of peptides, which will have less impact on the enrichment process of the inner TiO2 shell. The yolk-shell structure and two types of porous nanostructures endowed this composite with a high surface area (156.58 m(2) g(-1)) and a large pore volume (0.37 cm(3) g(-1)). Owing to the high surface area and combined properties of TiO2 and NiO, the Fe3O4@ H-TiO2@f-NiO microspheres showed a better performance for phosphopeptide enrichment than the same material without NiO nanosheets (Fe3O4@H-TiO2). According to the LC-MS/MS results, 972 unique phospho-peptides were identified from HeLa cell extracts with a high selectivity (91.9%) by Fe3O4@H-TiO2@f-NiO relative to 837 phosphopeptides (selectivity: 60.2%) by Fe3O4@H-TiO2. The results demonstrated that, compared with single-component metal oxides, composite metal oxides could enhance the selectivity and sensitivity for phosphopeptide enrichment.