文献类型：期刊文献

英文题名：Real-Time Quantitative PCR Analysis of the Expression Pattern of the Hypoglycemic Polypeptide-P Gene in Momordica charantia

作者：Wang, Yi-Shuai[1];Zeng, Xiang-Qing[2];Yang, Xu-Zhong[3];Liu, Wei[1];Li, Peng-Fei[1];Wang, Fu-Jun[2,3];Zhao, Jian[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China;[3]Zhejiang Reachall Pharmaceut Co Ltd, 209 West Hulian Rd, Dongyang City 322100, Peoples R China

年份：2019

卷号：10

期号：12

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000507342400097)】；

基金：This work was supported by Ph.D. Programs Foundation of Ministry of Education of China (20113107130001).

语种：英文

外文关键词：Momordica charantia; hypoglycemic polypeptide-P; qPCR; Western blotting; expression pattern

摘要：This study was designed to establish a real-time quantitative polymerase chain reaction (qPCR) method to rapidly and reliably analyze the hypoglycemic polypeptide-P gene expression pattern in Momordica charantia (MC) and to examine its expression changes in different MC accessions, harvesting seasons, and tissue types. The qPCR results were further verified by using Western blotting (WB). A total of 10 MCs with different accessions were collected and tested in this study. Among the tested accessions, RU5H showed the highest expression level of the polypeptide-P gene. The expression level of the polypeptide-P gene was not only season-related (with the highest in early July) but also tissue-related (with the highest in the seed tissue). In addition, the expression characteristic of the polypeptide-P gene was maturity-related, with the highest expression level in the tender MC. The WB results show that the transcription level of this gene shows an almost similar trend to the corresponding protein expression level. In conclusion, the established qPCR method can rapidly and effectively detect the expression levels of the polypeptide-P gene in MCs with different accessions; furthermore, various factors, including the accessions, harvesting seasons, and tissue types can affect the expression level.