文献类型：期刊文献

英文题名：In vitro toxicity evaluation of ultra-small MFe2O4 (M = Fe, Mn, Co) nanoparticles using A549 cells

作者：Zhao, Hongli[1,2];Chen, Zongyan[1,2];Tao, Liang[1,2];Zhu, Xiang[1,2];Lan, Minbo[1,2,3];Li, Zhen[4]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Soochow Univ, Sch Radiat Med & Protect, Collaborat Innovat Ctr Radiat Med, Jiangsu Higher Educ Inst, Suzhou 215123, Peoples R China

年份：2015

卷号：5

期号：84

起止页码：68454

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20153401196089);WOS:【SCI-EXPANDED(收录号:WOS:000359568400027)】；

基金：This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, contract No. 13ZR1412000).

语种：英文

外文关键词：High resolution transmission electron microscopy - X ray photoelectron spectroscopy - Superoxide dismutase - Manganese compounds - Cytotoxicity - Lipids - Nanomagnetics - Decomposition - Magnetite - X ray diffraction

摘要：As ferrite nanoparticles (MFe2O4) have been widely used in biomedical field, their safety evaluation has been paid great attention both in vitro and in vivo. In this paper, the ultra-small MFe2O4 (M = Fe, Mn, Co) nanoparticles with the average size less than 5 nm were prepared by thermal decomposition method and characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), high-resolution transmission electron microscopy (HRTEM), and vibrating sample magnetometry (VSM). The toxic effect on human lung epithelial A549 cells treated with MFe2O4 nanoparticles at the different concentrations was evaluated in vitro. Mitochondrial function (MTT assay), cellular morphology, reactive oxygen species (ROS), superoxide dismutase (SOD), membrane lipid peroxidation (LPO) and glutathione (GSH) were assessed as toxicity end points. The results showed that the cytotoxicity of ultra-small MFe2O4 nanoparticles was in a dose-and time-dependent manner. Moreover, ultra-small Fe3O4 nanoparticles were found to be nearly non-toxic in A549 cells, while MnFe2O4 nanoparticles exhibited cytotoxic effects, and CoFe2O4 nanoparticles exerted higher cytotoxic effects among the three studied particles at the same concentration.