文献类型：期刊文献

英文题名：Separation of ursodeoxycholic acid from its isomeric mixture using core-shell molecular imprinting polymer

作者：Xu Ziyun[1];Wan Junfen[1];Liang Shuang[1];Cao Xuejun[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：41

期号：3

起止页码：280

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20083311459173);WOS:【SCI-EXPANDED(收录号:WOS:000259711800011)】；

语种：英文

外文关键词：ursodeoxycholic acid; chenodeoxycholic acid; core-shell molecular imprinting polymers

摘要：In order to separate ursodeoxycholic acid (UDCA) from its isomeric mixture, the molecular imprinting polymers (MIPs) were synthesized by using core-shell emulsion polymerization. In the porous imprinting polymer, ursodeoxycholic acid was used as imprinting molecule, acrylamide (AM) and a-methacrylic acid (MAA) were functional monomers, and CaCO3 was used for the porogen in the polymerization to obtain large pore. Characterization of the MIP structure with I R spectra demonstrated the expected MIPs. Through adsorption and selectivity assays. AM as the functional monomer showed better separation efficiency than MAA, and nonspecific and specific adsorption capacities of MIP with AM were 43.52 and 13.93 mg/g, respectively. The separation factor of MIP with AM for UDCA was 2.20. Furthermore, MIP with AM could be applied to separate UDCA from the isomeric mixture by column chromatography successfully. (C) 2008 Elsevier B.V. All rights reserved.