文献类型：期刊文献

英文题名：Preparation of hemoglobin-loaded nano-sized particles with porous structure as oxygen carriers

作者：Zhao, Jian[1]; Liu, Chang-Sheng[1]; Yuan, Yuan[1]; Tao, Xin-Yi[2]; Shan, Xiao-Qian[1]; Sheng, Yan[1]; Wu, Fan[1]

机构：[1]E China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2007

卷号：28

期号：7

起止页码：1414

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20065210333848);WOS:【SCI-EXPANDED(收录号:WOS:000243773200010)】；

语种：英文

外文关键词：nanoparticles; hemoglobin; encapsulation efficiency; pore-connecting efficiency; oxygen-carrying capacity; circulation time

摘要：Hb (hemoglobin)-loaded particles (HbP) encapsulated by a biodegradable polymer used as oxygen carrier were prepared. A modified double emulsion and solvent diffusion/evap oration method was adopted. All experiments were performed based on two types of biodegradable polymers, poly(epsilon-caprolactone) (PCL) and poly(epsilon-caprolactone-ethylene glycol) (PCL-PEG). The biodistribution and the survival time in blood of the particles were investigated in a mouse model. Encapsulation efficiency and pore-connecting efficiency were evaluated by a novel sulfocyanate potassium method. The influence of process parameters on the particle size and pore-connecting efficiency (PCE%) of nanoparticles have been discussed. The prepared conditions: solvent, external aqueous phase, pressure were discussed. The system utilizing dichloromethane (DCM)/ethyl acetate (EA) as a solvent with an unsaturated external aqueous phase yielded the highest encapsulation efficiency (87.35%) with a small mean particle size (153 nm). The formation of porous channels was attributed to the diffusion of solvent. The PCE% was more sensitive to the rate of solvent diffusion that was obviously affected by the preparation temperature. The PCE% reached 87.47% when PCL-PEG was employed at 25 degrees C. P-50 of HbP was 27 mmHg, which does not seem to be greatly affected by the encapsulation procedure. In vivo, following intravenous injection of 6-coumarin labeled HbP, the major organ accumulating Hb-loaded particles was the liver. The half-life of nano-sized PCL HbP was 3.1 times as long as the micro-sized PCL HbP. Also, Nano-sized as well as a PEGylated surface on HbP is beneficial for prolonged blood residence (7.2 fold increase). (c) 2006 Elsevier Ltd. All rights reserved.