文献类型：期刊文献

英文题名：Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis

作者：Yamaguchi, Yoshinori[2,3];Li, Zhenqing[1];Zhu, Xifang[4];Liu, Chenchen[1];Zhang, Dawei[1];Dou, Xiaoming[2]

机构：[1]E China Univ Sci & Technol, Grad Sch Sci, IPBM, Shanghai 200237, Peoples R China;[2]Univ Shanghai Sci & Technol, Engn Res Ctr Opt Instrument & Syst, Shanghai Key Lab Modern Opt Syst, Minist Educ, Shanghai 200093, Peoples R China;[3]Osaka Univ, Grad Sch Engn, Dept Appl Phys, Suita, Osaka 5650871, Japan;[4]Changzhou Inst Technol, Coll Photoelect Engn, Changzhou 213002, Peoples R China

年份：2015

卷号：10

期号：5

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000353887100020)】；

基金：This project was partly supported by Grant-in-Aid for Scientific Research (Houga) (No. 25600049 (Y.Y.)), JSPS, Japan. The authors also gratefully acknowledge support from National Natural Science Foundation of China (No. 21205078 and No. 61378060), Research Fund for the Doctoral Program of Higher Education of China (No. 20123120110002), and the Innovation Fund Project for Graduate Student of Shanghai (No. JWCXSL1401), China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (N-min) from the resolution length analysis.