文献类型：期刊文献

中文题名：链霉素抗性突变理性筛选avermectin高产菌株

英文题名：Streptomycin-resistant rational screening for high yield avermectins producing strain

作者：陶纯长[1,2];谌颉[1,2];郭美锦[1,2];郭元昕[1,2];储炬[1,2];庄英萍[1,2];张嗣良[1,2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]国家生化工程技术研究中心(上海),上海200237

年份：2002

卷号：27

期号：9

起止页码：521

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：Avermectin;除虫链霉菌;链霉素抗性;理性筛选

外文关键词：Avermectins; Streptomyces avermitilis ; Streptomycin resistance; Rational screening

摘要：为提高菌株avermectin的产量 ,本文以链霉素抗性为选择压力 ,对除虫链霉菌Streptomycesavermi tilis进行紫外诱变 (2 53 7nm ,30w ,照射时间 45s) ,得到在摇瓶发酵水平比出发菌株产量提高 2 5 %以上的 4株突变株 ,其中 2株提高水平达 30 %以上。实验表明了链霉素抗性突变与产抗生素突变之间的密切联系。同时 。
To screen high avermectin producing strain,streptomycin resistant Strptomyces avermitilis was treated with ultraviolet (253 7nm, 30w, 45s). Four mutants were obtained, their productivities increased by more than 25% including 2 mutants by more than 30%, Results showed that there were close relationships of mutations between streptomycin resistance and antibiotic productivity, and the mechanism was also discussed.