文献类型：期刊文献

英文题名：Fitness of Chassis Cells and Metabolic Pathways for L-Cysteine Overproduction in Escherichia coli

作者：Liu, Han[1];Wang, Yu[1];Hou, Yehua[1];Li, Zhimin[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2020

卷号：68

期号：50

起止页码：14928

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20205109663014);WOS:【SCI-EXPANDED(收录号:WOS:000600204700020)】；

基金：This study was supported by the China National Key Research and Development Program (Grant No. 2019YFA0904300) and the Fundamental Research Funds for the Central Universities (22221818014).

语种：英文

外文关键词：Escherichia coli; l-cysteine; metabolic engineering; chassis cells

摘要：L-Cysteine is a ubiquitous and unique sulfur-containing amino acid with numerous applications in agricultural and food industries. The efficient production of L-cysteine via microbial fermentation has received a great deal of attention. In this study, the fitness of different Escherichia coli K-12 strains harboring plasmid pLH03 was investigated. The enhancement of the precursor synthetic pathway and thiosulfate assimilation pathway resulted in the good performance of the E. coli BW25113 strain. The expression levels of synthetic pathway genes were optimized by two constitutive promoters to assess their effects on cysteine production. In conjunction, the main degradation pathway genes were also deleted for more efficient production of cysteine. L-Cysteine production was further increased through the manipulation of the sulfur transcription regulator cysB and sulfur supplementation. After process optimization in a 1.5 L bioreactor, LH2A1M0B Delta YTS-pLH03 [BW25113 Ptrc2-serA Ptrc1-cysMPtrc-cysB Delta yhaM Delta tnaA Delta sdaA-(pLH03)] accumulated 8.34 g/L cysteine, laying a foundation for application in the cysteine fermentation industry.