文献类型：期刊文献

英文题名：Chitooligosaccharides display anti-tumor effects against human cervical cancer cells via the apoptotic and autophagic pathways

作者：Zhao, Mengyao[1];Gu, Liming[1];Li, Yun[1];Chen, Shumin[1];You, Jiangshan[1];Fan, Liqiang[1];Wang, Yudong[2];Zhao, Liming[1,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Dept Gynecol, Shanghai 200030, Peoples R China;[3]SCICBT, Shanghai 200237, Peoples R China

年份：2019

卷号：224

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20193307324071);WOS:【SCI-EXPANDED(收录号:WOS:000484001200033)】；

基金：This work was financially supported by "Shu Guang" project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (grant number 15SG28), the China Postdoctoral Science Foundation (grant number 2017M621392), the Shanghai PuJiang Program (grant number 18PJ1401900), the Fundamental Research Funds for the Central Universities (grant number 222201814036) and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, ECUST (ZDXM2019).

语种：英文

外文关键词：Chitooligosaccharides; Enzyme-membrane coupling reactor; Apoptosis; ROS production; Autophagy

摘要：Gynecological cancers are the most commonly diagnosed forms of cancer among the female population. Chitooligosaccharides (COS)-hydrolysis products from chitosan-display high bioavailability, high water solubility, and low molecular weight properties. Here, we investigated the influence of COS on 11 gynecological tumor cell types, and subsequently elucidated molecular mechanisms through which the observed inhibition occurred. Initially, we used a controllable enzyme-membrane coupling reactor system to obtain COS with a high degree of polymerization; the yield of high-degree-polymerized COS (DP 5-12) obtained with this reactor system accounted for similar to 75% yields (w/w). Using these COS materials, cell line assays showed that COS elicited the most significant anti-tumor activity against C33A cells, with anti-tumor mechanisms related to oxidative stress, as well as activation of intrinsic mitochondrial apoptosis and autophagic signaling. Thus, we provide experimental evidence to demonstrate how the enzyme-membrane coupling reactor system can generate COS that exert bioactivity against gynecological cancers.