文献类型：期刊文献

英文题名：Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis

作者：Lou, Tengfei[1];Chen, Kai[2,3,4,5];Luo, Qiyu[1];Liu, Changsheng[2,3,4,5];Yuan, Yuan[2,3,4,5];Fan, Cunyi[1]

机构：[1]Shanghai Sixth Peoples Hosp, Orthopaed Dept, Shanghai 200233, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2022

卷号：26

期号：1

外文期刊名：BIOMATERIALS RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000905961900001)】；

基金：T. Lou. and K. Chen. contributed equally to this work.This work was supported by the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the Frontiers Science Center for Materiobiology and Dynamic Chemistry (No. JKVD1211002), the National Natural Science Foundation of China (Nos. 31971264, 82172422, and 8217090787), the Key Project of National Natural Science Foundation of China (No. 81830076), and the National key R & D Program of China (No. 2021YFC2400801).

语种：英文

外文关键词：In situ precipitated CaP nanoparticles; Nanocomposite hydrogels; Bone adhesion; Stretchability; Dynamic distraction osteogenesis; Mechanical stimulation

摘要：Background: Distraction osteogenesis (DO) is an efficacious but lengthy procedure to reconstruct segmental bone defects under the principle of tension-stress, during which the periosteum-mediated mechanical stimulation plays a pivotal role. Inspired by the dynamic process of DO and the mechanical stimulation of periosteum, a new design of bionic periosteum was developed to simulate the mechanical transduction of natural periosteum for the application in DO procedure. Methods: In this study, an injectable organic-inorganic hybrid hydrogel was developed based on a novel combination of the PEGylated poly (glycerol sebacate) (PEGS) polymer network and in situ formed CaP nanoparticles (ICPNs). Rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured and tested in vitro to evaluate biocompatibility, cell adhesion, proliferation, and pro-osteogenic and pro-angiogenic activity. In vivo experiments were conducted in the rat tibial model of distraction osteogenesis. Results: The developed nanocomposite hydrogels exhibited excellent injectability, robust bone adhesion, superior stretchability, and enhanced osteogenic activity. The results of in vitro and in vivo studies showed that PEGS/ICPN hydrogels could promote new bone formation and mineralization during the dynamic distraction process through the synergistic effects of angiogenesis and osteogenesis. Conclusions: This periosteum-inspired nanocomposite hydrogel represents a mechanobiology approach for effectively restoring large bone defects through the dynamic DO process.