文献类型：期刊文献

英文题名：Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis

作者：Liu, Chenchen[1,2];Yamaguchi, Yoshinori[2,3];Sekine, Shinichi[4,5];Ni, Yi[2];Li, Zhenqing[1];Zhu, Xifang[6];Dou, Xiaoming[2,6]

机构：[1]Univ Shanghai Sci & Technol, Engn Res Ctr Opt Instrument & Syst, Shanghai, Peoples R China;[2]E China Univ Sci & Technol, Grad Sch Sci, Inst Photon & Biomed, Shanghai 200237, Peoples R China;[3]Osaka Univ, Grad Sch Engn, Dept Appl Phys, Osaka, Japan;[4]Osaka Univ, Osaka Univ Dent Hosp, Div Special Care Dent, Osaka, Japan;[5]Osaka Univ, Osaka Univ Grad Sch Dent, Dept Prevent Dent, Osaka, Japan;[6]ChangZhou Inst Technol, Sch Optoelect Engn, Changzhou, Jiangsu, Peoples R China

年份：2016

卷号：39

期号：5

起止页码：986

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20160401834721);WOS:【SCI-EXPANDED(收录号:WOS:000372280600024)】；

基金：This project was partly supported by Grand-in-Aid for Scientific Research (No. 25600049 (Y. Y.), A15H038270(Y. Y)), JSPS, Japan and The Innovation Fund Project for Graduate Student of Shanghai (No. JWCXSL1401), China.

语种：英文

外文关键词：Capillary polymer electrophoresis; Clinical diagnosis; Internal standard method; Oral bacteria; Quantitative analysis

摘要：Capillary polymer electrophoresis is identified as a promising technology for the analysis of DNA from bacteria, virus and cell samples. In this paper, we propose an innovative capillary polymer electrophoresis protocol for the quantification of polymerase chain reaction products. The internal standard method was modified and applied to capillary polymer electrophoresis. The precision of our modified internal standard protocol was evaluated by measuring the relative standard deviation of intermediate capillary polymer electrophoresis experiments. Results showed that the relative standard deviation was reduced from 12.4-15.1 to 0.6-2.3%. Linear regression tests were also implemented to validate our protocol. The modified internal standard method showed good linearity and robust properties. Finally, the ease of our method was illustrated by analyzing a real clinical oral sample using a one-run capillary polymer electrophoresis experiment.