文献类型：期刊文献

英文题名：Rhodamine-based fluorescent off-on sensor for Fe3+ - in aqueous solution and in living cells: 8-aminoquinoline receptor and 2: 1 binding

作者：Huang, Junhai[1,2,3];Xu, Yufang[1,2];Qian, Xuhong[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[3]Shanghai Inst Pharmaceut Ind, Zhangjiang R&D Ctr, Shanghai 200040, Peoples R China

年份：2014

卷号：43

期号：16

起止页码：5983

外文期刊名：DALTON TRANSACTIONS

收录：;EI(收录号：20141417542813);WOS:【SCI-EXPANDED(收录号:WOS:000333525500005)】；

基金：This work was financially supported by the National Basic Research Program of China (973 Program, 2010CB126100), the National Natural Science Foundation of China (Grant 21236002), the National High Technology Research and Development Program of China (863 Program 2011AA10A207), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Chromophores - Metals - Bioimaging - Fluorescence - Metal ions - Solutions - Nuclear magnetic resonance spectroscopy

摘要：8-Aminoquinoline (8-AQ) and 2-aminopyridine (AmPyr) both as ionophoric agents were robustly used in development of a cation sensor. To evaluate the effect of complexation with cations, flexible 2-aminopyridine and rigid 8-AQ moiety were directly introduced into rhodamine chromophore and two candidate fluorescent sensors were constructed successfully. Rh-AQ with a rigid 8-AQ fragment exhibited turn-on fluorescence and color responses to Fe3+ ion over other metal ions related to biology in aqueous solution. The sensor Rh-AmPyr with a flexible 2-aminopyridine did not give positive responses to cations although with a similar binding cavity as on Rh-AQ. Furthermore, the 2 : 1 recognition mode of Rh-AQ with Fe3+ was proved according to the 1D and 2D COSY H-H NMR experiments. The live cell imaging experiments demonstrated that Rh-AQ could be successfully applied as a bioimaging agent for monitoring Fe3+ in living cells.