文献类型：期刊文献

英文题名：Microfluidic reactor with immobilized enzyme-from construction to applications: A review

作者：Zhang, Han[1];Bai, Yunpeng[1];Zhu, Ning[2];Xu, Jianhe[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Nanjing 211800, Peoples R China

年份：2021

卷号：30

起止页码：136

外文期刊名：CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录：;EI(收录号：20210809975494);WOS:【SCI-EXPANDED(收录号:WOS:000651052100015)】；

语种：英文

外文关键词：Microfluidic immobilized enzyme reactor; Immobilization strategies; Biocatalysis; Bioconversion; Bioanalysis

摘要：Microfluidic, as the systems for using microchannel (micron- or sub-micron scale) to process or manipulate microflow, is being widely applied in enzyme biotechnology and biocatalysis. Microfluidic immobilized enzyme reactor (MIER) is a tool with great value for the study of catalytic property and optimal reaction parameter in a flourishing and highly producing manner. In view of its advantages in efficiency, economy, and addressable recognition especially, MIER occupies an important position in the investigation of life science, including molecular biology, bioanalysis and biosensing, biocatalysis etc. Immobilization of enzymes can generally improve their stability, and upon most occasions, the immobilized enzyme is endowed with recyclability. In this review, the enzyme immobilization techniques applied in MIER will be discussed, followed by summarizing the novel developments in the field of MIER for biocatalysis, bioconversion and bioanalysis. The preponderances and deficiencies of the current state-of-the-art preparation ways of MIER are peculiarly discussed. In addition, the prospects of its future study are outlined. (C) 2020 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.