文献类型：期刊文献

英文题名：Label-Free Monitoring of Single Molecule Immunoreaction with a Nanopipette

作者：Yu, Ru-Jia[1];Ying, Yi-Lun[1];Hu, Yong-Xu[1];Gao, Rui[1];Long, Yi-Tao[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：89

期号：16

起止页码：8203

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20173404055131);WOS:【SCI-EXPANDED(收录号:WOS:000407988600003)】；

基金：This research was supported by the National Natural Science Foundation of China (21421004, 21327807, 21505043, 21711530216), the Programme of Introducing Talents of Discipline to Universities (B16017) and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003, 222201714012).

语种：英文

外文关键词：Proteins - Solutions - Molecular dynamics

摘要：The nanopipette has been employed for the single molecule analysis due to its advantage of easy fabrication and controllable diameter. Herein, we present that the single molecule immunoreaction could be monitored by using the quartz nanopipette through the discrimination of characteristic blockade current, which reflect the intrinsic character of the individual unlabeled protein molecules due to its heterogeneous motion in solution. Our methods show the ability to monitor the immunoreaction between single alpha-fetal protein (AFP) and its specific antibody in aqueous solution without any labeling. Our studies may open a new door to comprehensively understand the single molecule immunoreaction, which gain more insight into the molecular dynamic of elethentary steps.