文献类型：期刊文献

中文题名：细胞生长和非生长相结合的拟干酪乳杆菌高效生产L-乳酸研究

英文题名：Combination of Cell Growth Dependent and Independent Modes for Efficient L-Lactic Acid Production by Lactobacillus paracasei

作者：张悦[1,2];田锡炜[1];庄英萍[1,2,3]

机构：[1]华东理工大学生物工程学院,上海200237;[2]华东理工大学发酵工程实验教学示范中心,上海200237;[3]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2021

卷号：42

期号：3

起止页码：77

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;EI(收录号：20241015701218);北大核心:【北大核心2020】；

基金：国家重点研发计划(2017YFB0309302);自然科学基金青年项目(31700038);中央高校科研基本业务费(WF1814032)。

语种：中文

中文关键词：拟干酪乳杆菌;乳酸;细胞生长和非生长相结合;静息细胞;高密度发酵

外文关键词：Lactobacillus paracasei;lactic acid;combination of cell growth dependence and independence;resting cell;high density fermentation

摘要：本研究以拟干酪乳杆菌发酵生产L-乳酸为研究对象,通过对最适初始葡萄糖浓度以及稳定期静息细胞的代谢能力进行研究,开发了基于细胞生长和非生长相关组合的高细胞密度L-乳酸生产策略。研究结果表明,140 g/L初始葡萄糖浓度下,细胞能够快速生长而且将葡萄糖完全耗尽生产L-乳酸。此外,在发酵结束后6~7 h之内,细胞仍然能够维持非常高的代谢活性。最后,在此基础上开发的高细胞密度生产策略,能够使细胞在正常批发酵结束后高密度发酵阶段的产率较常规发酵阶段提升1.4倍,而且L-乳酸转化率也明显提升,达到0.985 g/g。本研究通过过程策略能够有效延长细胞高速率生产L-乳酸的周期,从而提升L-乳酸生产效率,同时也在一定程度上能够有效缩短发酵过程所需的辅助时间,包括种子制备和细胞生长,因此有望应用于工业乳酸的高效生产。
In this study,on the basis of L-lactic acid production by Lactobacillus paracasei,a high cell density strategy with the combination of cell growth dependent and independent modes was developed through investigating the optimal initial glucose concentration and the metabolic capacity of resting cells.The results indicated that the cells grew well under 140 g/L of initial glucose concentration,and all the glucose could be rapidly exhausted to produce L-lactic acid.Moreover,the cells could still maintain high metabolic activity within 6~7 h after the end of fermentation.Finally,based on the above results,the L-lactic acid productivity of high cell density phase was enhanced by 1.4-fold compared with that of normal batch fermentation,and the L-lactic acid yield was also improved significantly to 0.985 g/g.This study extended the highly efficient production period so as to improve the production efficiency of L-lactic acid.Otherwise,the auxiliary time required in the fermentation process could be effectively reduced,including seed preparation and cell growth.Thus,it would be potentially applied for future industrial lactic acid production.