文献类型：期刊文献

中文题名：脂肪酶产生菌Candida rugosa产酶条件研究

英文题名：Studies on Lipase Production from Candida rugosa

作者：宋庆训[1];林金萍[1];戎一平[1];魏东芝[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,生物化学研究所,上海200237

年份：2001

卷号：17

期号：1

起止页码：101

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家"九五"攻关项目!(96 C0 3 0 2 0 5 );上海市科委攻关项目!(98431912 2 );国家自然科学基金!(2 970 6 0 0 3)资助项目&&

语种：中文

中文关键词：CAnDiDARugosA;脂肪酶;发酵条件优化;假丝酵母;

外文关键词：Candida rugosa, lipase, optimization of fermentation condition

摘要：We studied some factors affecting the lipase production from candida rugosa, they mainly included medium compositions and culture condition.The result showed that the optimal medium compositions for lipase production are 0.1% glucose 4.0% olive oil (carbon source),0.3% NH 4NO 3(nitrogen source),1.2%K 2HPO 4 and 0.4%MgSO 4·7H 2O.And the optimal culture condition is initial pH6.5,temperature 30℃,agitation 180r/min and time 60h. As a result, and the lipase activity could reach 19.5u/mL.Meanwhile we found that the surfactant could be helpful to the lipase production, and the optimal surfactant concentration was 0.03% GPE.The lipase activity was improved by more than 170% after we optimized the medium compositions and culture condition.While in a 5L fermentator, the lipase activity of fermentation broth could reach 33.5u/mL within 48 hours.
We studied some factors affecting the lipase production from candida rugosa, they mainly included medium compositions and culture condition.The result showed that the optimal medium compositions for lipase production are 0.1% glucose 4.0% olive oil (carbon source),0.3% NH 4NO 3(nitrogen source),1.2%K 2HPO 4 and 0.4%MgSO 4·7H 2O.And the optimal culture condition is initial pH6.5,temperature 30℃,agitation 180r/min and time 60h. As a result, and the lipase activity could reach 19.5u/mL.Meanwhile we found that the surfactant could be helpful to the lipase production, and the optimal surfactant concentration was 0.03% GPE.The lipase activity was improved by more than 170% after we optimized the medium compositions and culture condition.While in a 5L fermentator, the lipase activity of fermentation broth could reach 33.5u/mL within 48 hours.