文献类型：期刊文献

英文题名：Characterization of the bagremycin biosynthetic gene cluster in Streptomyces sp. Tu 4128

作者：Ye, Jiang[1,2];Zhu, Yunxia[2];Hou, Bingbing[1,2];Wu, Haizhen[1,2];Zhang, Huizhan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai, Peoples R China

年份：2019

卷号：83

期号：3

起止页码：482

外文期刊名：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000458668300011)】；

基金：This work was supported by the National Natural Science Foundation of China [3120026].

语种：英文

外文关键词：Streptomyces sp. Tu 4128; bagremycins; biosynthetic gene cluster; antibiotics

摘要：Bagremycin A and bagremycin B isolated from Streptomyces sp. Tu 4128 have activities against Gram-positive bacteria, fungi and also have a weak antitumor activity, which make them have great potential for development of novel antibiotics. Here, we report a draft genome 8,424,112 bp in length of S. sp. Tu 4128 by Illumina Hiseq2000, and identify the bagremycins biosynthetic gene cluster (BGC) by bioinformatics analysis. The putative bagremycins BGC includes 16 open reading frames (ORFs) with the functions of biosynthesis, resistance and regulation. Disruptions of relative genes and HPLC analysis of bagremycins production demonstrated that not all the genes within the BGC are responsible for the biosynthesis of bagremycins. In addition, the biosynthetic pathways of bagremycins are proposed for deeper inquiries into their intriguing biosynthetic mechanism.