文献类型：期刊文献

中文题名：金色链霉菌培养条件优化研究

英文题名：Optimization of the Cultivation Condition with Streptomyces aureofaciens

作者：扶教龙[1];张明[1];樊涛[1];储炬[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心,上海200237

年份：2004

卷号：30

期号：1

起止页码：43

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2004138091822);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：金色链霉菌;金霉素;发酵;优化;培养条件

外文关键词：Streptomyces aureofaciens; chlortetracycline; fermentation; optimization

摘要：金色链霉菌摇瓶培养适宜的接种量为3.2%、接种时间为25h、温度在28°C左右。4g/L的硫酸铵和0.043g/L无机磷有利于产物金霉素(CTC)合成。酵母膏的加入对CTC的合成有一定促进作用,以3g/L的效果较好。在50L发酵罐中,较低浓度的无机磷对CTC发酵有利;酵母膏的加入量以4.2g/L最佳,产物效价比酵母膏加入量为2.8g/L和5.6g/L时高3.1%和9.7%,优化发酵过程的CTC产率提高了18%。
The optimal quantity and age of inoculation and temperature is 3.2%、25 h and around 30 °C respectively in CTC fermentation in flask. The effects of 4 g/L (NH_4)_2SO_4 and 0.043 g/L inorganic phosphate on CTC biosynthesis are better than the others. The supplement of yeast extract benefits to the fermentation and the concentration of 3 g/L is better. The results of CTC fermentation in 50 L fermentor (indicate) the titer of CTC at 0.05 g/L KH_2PO_4 is increased by 6.7% compared with 0.15 g/L. And 4.2 g/L yeast extract is the best to CTC biosynthesis, the CTC titer is higher by 3.1% and 9.7% than other two concentration at 2.8 g/L and 5.6 g/L. The CTC titer in optimized process increased by about 18%.