文献类型：期刊文献

英文题名：Protective Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone to PC12 Cells against Cytotoxicity Induced by Hydrogen Peroxide

作者：Su, Ming-Yuan[1];Huang, Hai-Ya[1];Li, Lin[2];Lu, Yan-Hua[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Unilever Res & Dev China, Shanghai 200335, Peoples R China

年份：2011

卷号：59

期号：2

起止页码：521

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20110413617580);WOS:【SCI-EXPANDED(收录号:WOS:000286176100008)】；

基金：This work was supported by the National Natural Science Foundation of China (30870253) and partially supported by the Shanghai-Unilever Research and Development Fund (09540715800) and Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：2 ',4 '-Dihydroxy-6 '-methoxy-3 ',5 '-dimethylchalcone; PC12 cells; oxidative stress; hydrogen peroxide; reactive oxygen species

摘要：Oxidative stress has been considered as a major cause of cellular injuries in various clinical abnormalities. One of the possible ways to prevent reactive oxygen species (ROS)-mediated cellular injury is dietary or pharmaceutical therapies to augment the endogenous antioxidant defense capacity. The present study found that 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), a chalcone isolated from the buds of Cleistocalyx operculatus, possessed cytoprotective activity in PC12 cells treated with H2O2. The results showed that DMC could effectively increase cell viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) reduction], decrease the cell apoptotic percentage [annexin V/propidium iodide (AV/PI) assay], prevent the membrane from damage [lactate dehydrogenase (LDH) release], scavenge ROS formation, reduce caspase-3 activity, and attenuate the decrease of mitochondrial membrane potential (MMP) in PC12 cells treated with H2O2. Meanwhile, DMC increased the catalytic activity of superoxide dismutase (SOD) and the cellular amount of glutathione (GSH), decreased the cellular amount of malondialdehyde (MDA), and decreased the production of lipid peroxidation in PC12 cells treated with H2O2.