文献类型：期刊文献

中文题名：不同处理方法对灵芝β-葡聚糖降解效果的比较研究

英文题名：A comparative study of different methods of degrading Ganoderma lingzhiβ-glucan

作者：秦秀[1,2];刘艳芳[2];张劲松[2];唐庆九[2];周帅[2];冯杰[2];王晨光[3];李德顺[2];韩伟[1]

机构：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心上海市新药设计重点实验室,上海200237;[2]上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点实验室,上海201403;[3]上海百信生物科技有限公司,上海201403

年份：2021

卷号：40

期号：9

起止页码：2495

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：上海市自然科学基金(20ZR1418700);上海市闵行区领军人才项目(201844)。

语种：中文

中文关键词：灵芝β-葡聚糖;β-葡寡糖;降解

外文关键词：Ganodermaβ-glucan;β-glucooligosaccharide;degradation

摘要：本研究采用酸法、碱法、酶法和微波法对灵芝β-葡聚糖进行降解,通过降解率、产物分子量变化、产物聚合度分布等指标比较了不同方法的降解效果。结果表明,微波法降解率高达94%,处理后产物的分子量明显降低,寡糖产物聚合度分布广。酶法降解率约为40%,寡糖产物中含有DP2-5的成分。酸法及碱法降解率低于20%,寡糖产物少。研究表明,与其他3种方法相比,微波法降解率高、产物丰富、操作条件易于控制,是一种简单、高效的降解灵芝β-葡聚糖、制备灵芝β-葡寡糖的方法。
Different methods were used to degrade Ganoderma lingzhiβ-glucan,and the degradation rate,molecular weight variation and polymerization degree distribution were used as indexes to compare the degradation effects.Results showed that the degradation rates of theβ-glucans treated by microwave,enzymatic and chemical methods were about 94%,40%and 20%,respectively.By use of microwave method,the molecular weight of degradation products significantly reduced,and the polymerization degree of oligosaccharides was widely distributed.The distribution of oligosaccharide polymerization degree inβ-glucan degradation products obtained through enzymatic treatment was narrow,and the oligosaccharide products under acid and alkali treatments were less.Among four tested methods,microwave exhibited the highest efficiency,and the operation was simple and controllable,being suitable for the preparation of Ganoderma lingzhiβ-glucooligosaccharide.