文献类型：期刊文献

中文题名：Molecular phylogenetic diversity of the microbial community associated with a high-temperature petroleum reservoir at an offshore oilfield

英文题名：Molecular phylogenetic diversity of the microbial community associated with a high-temperature petroleum reservoir at an offshore oilfield

作者：Li H[1,2];Yang SZ[1,2];Mu BZ[1,2];Rong ZF[1,2];Zhang J[1,2]

机构：[1]E China Univ Sci ＆ Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci ＆ Technol, Inst Bioengn, Shanghai, Peoples R China

年份：2007

卷号：21

期号：6

起止页码：33

中文期刊名：中国生物学文摘

外文期刊名：Chinese Biological Abstracts

语种：英文

中文关键词：遗传多样性;微生物群落;海上油田;高温陆地石油贮藏库;16S;rRNA

外文关键词：petroleum reservoir; 16S rRNA gene diversity; microbial commmunity; clone library RIBOSOMAL-RNA GENES; CONTINENTAL HIGH-TEMPERATURE; SULFATE-REDUCING BACTER/A; SPNOV.; DEEP SUBSURFACE; PCR; WATER; BIAS; BIOREMEDIATION; SUBTERRANEUS

摘要：The microbial community and its diversity in production water from a high-temperature, water-flooded petroleum reservoir of an offshore oilfield in China were characterized by 16S rRNA gene sequence analysis. The bacterial and archaeal 16S rRNA gene clone libraries were constructed from the comlnunity DNA and, using sequence analysis, 388 bacterial and 220 archaeal randomly selected clones were clustered with 60 and 28 phylotypes, respectively. The results showed that the 16S rRNA genes of bacterial clones belonged to the divisions Firmicutes, Thermotogae, Nitrospirae and Proteobacteria, whereas the archaeal library was dominated by methanogen-like rRNA genes （Methanothermobacter, Methanobacter, Methanobrevibacter and Methanococcus）, with a lower percentage of clones belonging to Thermoprotei. Thermophilic microorganisms were found in the production water, as well as mesophilic microorganisms such as Pseudomonas and Acinetobacter-like clones. The thermophilic microorganisms may be common inhabitants of geothermally heated specialized subsurface environments, which have been isolated previously from a number of high-temperature petroleum reservoirs worldwide. The mesophilic microorganisms were probably introduced into the reservoir as it was being exploited. The results of this work provide further insight into the composition of microbial communities of high-temperature petroleum reservoirs at offshore oilfields.