文献类型：期刊文献

英文题名：THE SURFACTIN AND LICHENYSIN ISOFORMS PRODUCED BY BACILLUS LICHENIFORMIS HSN 221

作者：Li, Yiming;Yang, Shizhong;Mu, Bozhong[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

年份：2010

卷号：43

期号：6

起止页码：929

外文期刊名：ANALYTICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000275436100004)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 50744016), the grant from the Ministry of Science and Technology of China (2007CB707801), and Shanghai Municipal Science and Technology Commission (071607014).

语种：英文

外文关键词：Bacillus licheniformis; Isoforms; Lichenysin; Lipopeptide; Surfactin

摘要：Surfactin and lichenysin are typical members of the lipopeptide family. Four surfactin and two lichenysin isoforms were isolated by Pre RP-HPLC from the cell-free broth of Bacillus licheniformis HSN 221 cultivated in YPD medium. The molecular weight of each isoform was determined by ESI-MS. The peptide part of each isoform was elucidated by Q-Tof MS/MS combined with HPLC, and the fatty acid part was analyzed by GC/MS. It showed that the four surfactin isoforms have the primary structures of anteiso(iso)C-13, isoC(14), anteiso(iso)C-15, anteiso(iso)C-15-surfactin-methyl ester, and the two lichenysin isoforms have those of nC(14) and anteiso(iso)C-15, respectively.