文献类型：期刊文献

英文题名：Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae

作者：Ma, Bin[1];Liu, Min[1];Li, Zhen-Hai[1];Tao, Xinyi[1];Wei, Dong-Zhi[1];Wang, Feng-Qing[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：67

期号：31

起止页码：8590

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20193307317293);WOS:【SCI-EXPANDED(收录号:WOS:000480498000019)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 31500043), the Natural Science Foundation of Shanghai (No. 19ZR1473000), the General Project of Beijing Municipal Education Commission (No. SQKM201311417004), the Fundamental Research Funds for the Central Universities (No. 22221818014), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：patchoulol; sesquiterpene; metabolic engineering; Saccharomyces cerevisiae; terpenoids

摘要：Patchoulol, a natural sesquiterpene compound, is widely used in perfumes and cosmetics. Several strategies were adopted to enhance patchoulol production in Saccharomyces cerevisiae: (i) farnesyl pyrophosphate (FPP) synthase and patchoulol synthase were fused to increase the utilization of FPP precursor; (ii) expression of the limiting genes of the mevalonate pathway was enhanced; (iii) squalene synthase was weakened by a glucose-inducible promoter of HXT1 (promoter for hexose transporter) to reduce metabolic flux from FPP to ergosterol; and (iv) farnesol biosynthesis was inhibited to decrease the consumption of FPP. Glucose was used to balance the trade-off between the competitive squalene and patchoulol pathways. The patchoulol production was 59.2 +/- 0.7 mg/L in a shaken flask with a final production of 466.8 +/- 12.3 mg/L (20.5 +/- 0.5 mg/g dry cell weight) combined with fermentation optimization, which was 7.8-fold higher than the reported maximum production. The work significantly promoted the industrialization process of patchoulol production using biobased microbial platforms.