文献类型：期刊文献

英文题名：A strategy for evaluating antipyretic efficacy of Chinese herbal medicines based on UV spectra fingerprints

作者：Ni, Li-Jun[1];Zhang, Li-Guo[1];Hou, Jun[1];Shi, Wan-Zhong[2];Guo, Mei-Lan[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, POB 425,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Shuguang Hosp, Shanghai 200021, Peoples R China

年份：2009

卷号：124

期号：1

起止页码：79

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000268201200009)】；

基金：The authors would like to thank the National Ministry of Science and Technology of China for financially supporting this research (Contract No. 2006BAI09B07-10).

语种：英文

外文关键词：Chinese herbal medicine; Fingerprint-efficacy analysis; Candidate herbal drug selection; Canonical correlative analysis; Principal component analysis

摘要：Ethnopharmacological relevance: Quality analysis and control of Chinese herbal medicines (CHM) or herbal medicines (HM) are being more and more investigated based on fingerprint analysis, and there are also some researches on correlating fingerprints of CHM to their efficacy. Multi-component analysis methods together with fingerprints are considered potential useful tools to select candidate herbal drugs from extracts of herbs in pharmacological/bio-prospecting investigations. Aim of the study: To explore a strategy for evaluating efficacy strength of CHM samples based on their spectra fingerprints and validate it. Methodology: Radix bupleuri (RB), a typical Chinese medicinal herb for relieving exterior syndrome, and Flos lonicerae (FL), Fructus forsythiae (FF), and Radix isatidis (RI) that are widely applied Chinese herbs for heat clearing and detoxifying, were selected as herbal sources. The aqueous extracts, volatile oils and mixtures of the extracts and oils of the four herbs, plus Ibuprofen suspension (IS). Shuanghuanglian oral liquid (SHL), mixture of SHL and the volatile oils of FL and FF, were used for subject samples to do antipyretic experiments on rats. Ultraviolet spectra were used as the spectra fingerprints to represent chemical characteristics of the samples. Principal component analysis (PCA) and canonical correlation analysis (CCA) were adopted as evaluation tools to establish the correlation between pharmacological and spectra data, from which a spectral index for evaluating antipyretic effects of CHM samples was constructed. Furthermore, four compound samples were designed by mixing 50% volatile oils and 50% aqueous extracts of the four herbs with different ratios to validate the strategy. Results: Efficacy sequence of the 15 calibrating and 4 validating CHM samples, defined by the first canonical correlative variable U-1 of their UV spectra, was consistent with that given by pharmacological experiments. Conclusions: The strategy proposed in this study could be applied to evaluate efficacy strength of CHM and helpful for screening candidate herbal drugs from different herbs or prepared by different technologies. (C) 2009 Elsevier Ireland Ltd. All rights reserved.