文献类型：期刊文献

英文题名：An open tubular capillary electrochromatography column with porous inner surface for protein separation

作者：Liu, Haiyan[1];Li, Xiaohui[1];Huang, Lanqi[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：442

期号：2

起止页码：186

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;EI(收录号：20201908624452);WOS:【SCI-EXPANDED(收录号:WOS:000326064600010)】；

基金：The authors are grateful for financial support from the Excellent Youth Founding of East China University of Science and Technology (YJ0157104), the 973 Project from the Ministry of Science and Technology of the People's Republic of China (2011CB910404), and the National Natural Science Foundation of China (21075039).

语种：英文

外文关键词：Proteins; Open tubular capillary; electrochromatography; Porogen; Porous inner surface

摘要：An open tubular capillary electrochromatography (OTCEC) column using sole porogen to form porous inner surface has been developed. The porous layer was coated on the capillary inner wall by in situ polymerization in the presence of porogen. The results show that the columns using 1-propanol as sole porogen are appropriate for protein separation. It has higher separation efficiency than the column with the usual coporogen due to much more micropores and mesopores on the porous surface and a higher specific surface area. In addition, the sensitivity of the prepared OTCEC column was improved greatly compared with the dynamically coated capillary with polyvinylpyrrolidone. (C) 2013 Elsevier Inc. All rights reserved.