文献类型：期刊文献

中文题名：不同有机酸对粘质沙雷氏菌合成2,3-丁二醇的影响

英文题名：Effects of different organic acids on 2,3-butanediol production by Serratia marcescens

作者：张燎原[1];黄晓燕[1];孙建安[2];魏东芝[2];沈亚领[2]

机构：[1]福建农林大学,生物农药与化学生物学教育部重点实验室,福州350002;[2]华东理工大学,生物反应器国家重点实验室,鲁华生物技术研究所,上海200237

年份：2012

卷号：42

期号：4

起止页码：1

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：CSCD:【CSCD2011_2012】；

基金：福建省自然科学基金项目(2011J05048);福建省教育厅A类科技项目(K8011044)

语种：中文

中文关键词：粘质沙雷氏菌;2,;3-丁二醇;有机酸;融合表达分析

外文关键词：Serratia marcescens, 2,3-butanediol, organic acids, fusion expression

摘要：考察了不同的短链有机酸对粘质沙雷氏菌合成2,3-丁二醇的影响,结果表明乙酸、乳酸、丙酮酸、琥珀酸、延胡索酸和柠檬酸均能在一定程度上提高2,3-丁二醇的产量,其中乙酸的效果最为明显,在基础培养基中添加6 g/L乙酸,与对照相比,2,3-丁二醇的产量提高了91.06%,此外菌体干重也提高了58.28%。为了揭示其中的调控机制,构建了启动子:lacZ融合报告载体,lacZ活性测定显示六种有机酸均可提高报告基因β-半乳糖苷酶的表达,其中乙酸可提高β-半乳糖苷酶活性近4倍,暗示六种有机酸促进2,3-丁二醇的合成可能与诱导该合成途径相关基因的表达有关。
Effects of different short chain organic acids on 2, 3-butanediol production by Serratia marcescens were investigated. Results showed that acetate, lactate, pyruvate, succinate, fumarate and citrate could improve the yield of 2,3- butanediol. Among these organic acids, acetate of 6 g/L provided the increase of 91.06% for 2,3-butanediol yield and 58.28% for cell dry weight compared with the control. To elucidate the regulatory mechanism, a report plasmid with the promoter from 2,3-butanediol pathway and a lacZ gene was constructed. ^-galactosidase activities assay indicated that six organic aicds could improve the expression of lacZ gene. Approximately 4-fold β-galactosidase activities increase was observed in the presence of acetate under the control. These results showed six organic acids promoted 2,3-butanediol biosynthesis through inducing the expression of these genes involved 2,3-butanediol biosynthetic pathway in Serratia