文献类型：期刊文献

英文题名：Structural Analysis of the Lipopeptide Produced by the Bacillus subtilis Mutant R2-104 with Mutagenesis

作者：Meng, Yong[1,2];Zhao, Wei[1,2];You, Jia[1,2];Gang, Hong-Ze[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Ye, Ru-Qiang[1,2];Mu, Bo-Zhong[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

年份：2016

卷号：179

期号：6

起止页码：973

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20161302174786);WOS:【SCI-EXPANDED(收录号:WOS:000381157400006)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 21203063 and 51574125) and the 863 Program (Grant No. 2013AA064403).

语种：英文

外文关键词：Mutagenesis; Bacillus subtilis; Lipopeptide; Surfactin; Structural analysis

摘要：The lipopeptide and its homologues are a kind of the five major biosurfactants with prominent interfacial and biological activities. A suite of mutagenesis method was adopted to expose a wild lipopeptide-producing strain Bacillus subtilis HSO121 to improve lipopeptide yield, and a stable mutant named R2-104 with a 2.0-fold production of lipopeptide was obtained. Compared to that of the wild strain HSO121, the lipopeptide produced by R2-104 showed a similar surface activity, but the course profiles of lipopeptide production during cultivation were different, with the peak yield of 500 mg at about 9 h by R2-104, and 400 mg at about 5 h by HSO121. The constituent abundance of the lipopeptide homologues produced by R2-104 was also different from that by HSO121. Combined methods of ESI-MS, GC-MS and MS-MS were applied for structural characterization of lipopeptide homologues, and it showed that the lipopeptides produced by R2-104 and HSO121 were attributed to a surfactin family with different constituents. The dominant constituent of the surfactin family produced by R2-104 was anteiso C-15-surfactin with a relative content of 43.8 %, while the dominant one produced by HSO121was iso C-14-surfactin with a relative content of 33.1 %.