文献类型：期刊文献

英文题名：An miRISC-initiated DNA nanomachine for monitoring MicroRNA activity in living cells

作者：Yin, Bin-Cheng[1];Ye, Bang-Ce[1];Wang, Jin-Yu[1];Li, Hua-Dong[1];Ma, Pei-Qiang[1,2];Zhou, Ying[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China

年份：2023

卷号：220

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20224413052105);WOS:【SCI-EXPANDED(收录号:WOS:000899985800007)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grants 21822402, 22134003, 31730004, 22104037) , "Shuguang Program" supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission, and the Joint Funds of the Zhejiang Provincial Natural Science Foundation of China (Grant LHDMZ22H300008) .

语种：英文

外文关键词：Biosensor; DNA nanomachine; MicroRNA activity; MicroRNA-induced silencing complex; Gold nanoparticle

摘要：MicroRNAs (miRNAs) play an important role in post-transcriptional regulation of gene expression. However, methods to accurately detect miRNA activity in living cells are still limited. Here we developed a DNA nanomachine initiated by a miRNA-induced silencing complex (miRISC) for imaging miRNA activity in living cells. miRISC-mediated RNA cleavage reaction activated the DNA nanomachine by the specific cleavage of an RNA strand on the machine, resulting in autonomous movement of the walking leg around the AuNP surface with the release of a large number of fluorescently labeled DNA strands. The DNA nanomachine was successfully applied to detect miR-21 activity in three cell lines with different miR-21 expression profiles. We also demonstrated that terminal uridylyltransferase Tut4 knockdown by siRNA significantly increased the activity of let-7b miRNA, which further verifies the versatility of our DNA nanomachine. This new nanomachine has distinct advantages compared with reported methods for detecting miRNA activity, including simple operating procedures, short analysis time and sensitive signal output. Collectively, this work not only expands the application of the DNA nanomachine in the detection of miRNA activity, but also provides a promising tool for basic research in cell biology and development of clinical biomedicine.