文献类型：期刊文献

英文题名：Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable β-1,3-1,4-glucanase

作者：Luo, Zichen[1];Gao, Qiuqiang[1];Li, Xinliang[2];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Youtell Biotech Co, Shanghai 201201, Peoples R China

年份：2014

卷号：173

期号：2

起止页码：562

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20142017712477);WOS:【SCI-EXPANDED(收录号:WOS:000336492000019)】；

基金：This research was supported by the China Postdoctoral Science Foundation (2012M520850) and the Fundamental Research Funds for the Central Universities of China (WF0913005/1214025).

语种：英文

外文关键词：beta-1,3-1,4-glucanase; LicB; Clostridiumthermocellum; Secretive expression; Thermostability

摘要：beta-1,3-1,4-glucanase is a widely used enzyme in brewing and in animal feed processing. To produce the bacterial enzyme at an industrial scale, the enzyme should be able to be secreted from microbial cells into fermentation broth and be stable in different conditions. In this study, the LicB gene encoding beta-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum was secretively expressed in a secretive strain, Bacillus subtilis WB800, with eight extracellular protease deletion which made LicB expressed obviously and reached 1.18 U/g cell mass. The secreted beta-1,3-1,4-glucanase was found to be active from 40 A degrees C to 80 A degrees C and achieved the optimal activity at 80 A degrees C. The enzyme also has a wide pH range (pH 4-11). The most common metal ions and chemicals were found to be inert on its activity. The property of LicB-encoded beta-1,3-1,4-glucanase and its efficient secretive expression makes it a potential enzyme for industrial production and application.