文献类型：期刊文献

英文题名：Anti-Hyperuricemic, Nephroprotective, and Gut Microbiota Regulative Effects of Separated Hydrolysate of α-Lactalbumin on Potassium Oxonate- and Hypoxanthine-Induced Hyperuricemic Mice

作者：Xie, Dewei[1];Shen, Yaling[1];Su, Erzheng[2];Du, Lei[1];Xie, Jingli[1,3];Wei, Dongzhi[1,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Dept Food Sci & Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Nanjing Forestry Univ, Coll Light Ind & Food Engn, Nanjing 210037, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai 200237, Peoples R China

年份：2023

卷号：67

期号：1

外文期刊名：MOLECULAR NUTRITION & FOOD RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000883473100001)】；

基金：This work was supported by National Key Research and Development Program of China (No. 2020YFA0907800), China; Natural Science foundation of Shanghai (No. 21ZR1416200), China.

语种：英文

外文关键词：anti-hyperuricemia; gut microbiota; hydrolysate; nephroprotection; alactalbumin

摘要：Scope: This study aims to investigate the anti-hyperuricemic and nephroprotective effects and the potential mechanisms of the separated gastrointestinal hydrolysates of adactalbumin on hyperuricemic mice. Methods and results: The gastrointestinal hydrolysate of alpha-lactalbumin, the hydrolysate fraction with molecular weight (MW) < 3 kDa (LH-3k), and the fragments with smallest MW among LH-3K harvested through dextran gel chromatography (F5) are used. Hyperuricemia mice are induced via daily oral gavage of potassium oxonate and hypoxanthine. F5 displays the highest in vitro xanthine oxidase (XO) inhibition among all the fractions separated from LH-3k. Oral administration of F5 significantly reduces the levels of serum uric acid (UA), creatinine, and urea nitrogen. F5 treatment could ameliorate kidney injury through alleviating oxidative stress and inflammation. F5 alleviates hyperuricemia in mice by inhibiting hepatic XO activity and regulating the expression of renal urate transporters. Gut microbiota analysis illustrates that F5 administration increases the abundance of some SCFAs producers, and inhibits the growth of hyperuricemia and inflammation associated genera. LH-3k exhibits similar effects but does not show significance as those of the F5 fraction. Conclusion: The anti-hyperuricemia and nephroprotective functions of F5 are mediated by inhibiting hepatic XO activity, ameliorating oxidative stress and inflammation, regulating renal urate transporters, and modulating the gut microbiota in hyperuricemic mice.