文献类型：期刊文献

英文题名：The improvement of adenovirus vector production by increased expression of coxsackie adenovirus receptor

作者：Liu, Xuping[1];Wang, Yigang[1];Niu, Hongxing[1];Zhang, Xu[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：31

期号：7

起止页码：939

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20092712161712);WOS:【SCI-EXPANDED(收录号:WOS:000267056600003)】；

基金：This work was partially funded by the National Science Foundation of China (NSFC projects no. 20706016).

语种：英文

外文关键词：Adenovirus; CAR; Cell density effect; Cell-specific productivity; HEK 293 cells

摘要：Adenoviruses are promising vectors for gene therapy. The production of adenoviral vectors (AdV), however, is limited by the cell density effect, namely when cell infection is performed at above 10(6) cells/ml, a drop in cell-specific adenovirus productivity occurs. Our results also show that the coxsackie adenovirus receptor (CAR) plays an important role in AdV production. CAR expression of infected cells varied with culture time and the cell-specific AdV productivity dropped rapidly along with decreased CAR expression. Furthermore, CAR expression of cells was maintained at a high level by replacing the medium or supplementing it with trichostatin A, which could improve the cell-specific productivity. Thus, a higher CAR expression level at infection time could improve cell-specific AdV productivity at high cell densities.