文献类型：期刊文献

英文题名：Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401

作者：Xu, Guo-Qian[1];Chu, Ju[1];Zhuang, Ying-Ping[1];Wang, Yong-Hong[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, Natl Engn Ctr Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：38

期号：2

起止页码：189

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000252915700008)】；

语种：英文

外文关键词：L(+)-lactic acid; steepest ascent method; response surface analysis; vitamin; cell viability

摘要：The effects of various vitamin supplements, vitamin B-1, B-2, B-5, B-6, and H on the fermentation of Lactobacillus paracasei NERCB, 0401 for production of lactic acid were investigated. Vitamin B, and H were found to be the determinant supplements for the fermentation process. The optimal addition of vitamin supplements in defined medium were experimentally studied and found be 0.053 mg/L vitamin B-1, 0.01 mg/L vitamin B-2, 4.0 mg/L vitamin B-5, 0.2mg/L vitamin B-6, and 0.075 mg/L vitamin H. The concentration of lactic acid was 92% greater under the optimal addition of the vitamin components than that without vitamins addition. Compared to the fermentation without vitamins supplement addition, the death rate of L. paracasei under the optimal vitamin addition decreased remarkably, with maximum extent by 53.5% at 24h. The possible mechanism of enhancement of lactic acid production was elucidated by the time course analysis of the specific activities of phosphofructokinase, lactate dehydrogenase, pyruvate dehydrogenase complex and pyruvate carboxylase during fermentation processes. (c) 2007 Elsevier B.V. All rights reserved.