文献类型：期刊文献

英文题名：Occurrence and molecular characterization of Cryptosporidium spp. and Enterocytozoon bieneusi in dairy cattle, beef cattle and water buffaloes in China

作者：Ma, Jingbo[1];Li, Pei[1];Zhao, Xiaoping[2];Xu, Hailing[1];Wu, Wenxian[1];Wang, Yuanfei[1];Guo, Yaqiong[1];Wang, Lin[1];Feng, Yaoyu[1];Xiao, Lihua[3]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Yueyang Ctr Anim Dis Control & Prevent, Yueyang 414000, Hunan, Peoples R China;[3]Ctr Dis Control & Prevent, Div Foodbome Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA

年份：2015

卷号：207

期号：3-4

起止页码：220

外文期刊名：VETERINARY PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000349271100004)】；

基金：This work was supported by National Natural Science Foundation of China (31110103901 and 31229005), National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and Fundamental Research Funds for the Central Universities, China (No. WB1214073).

语种：英文

外文关键词：Cryptosporidium; Enterocytozoon bieneusi; Cattle; SSU rRNA gene; ITS; Nested PCR

摘要：Cryptosporidium spp. and Enterocytozoon bieneusi are important protists in a wide range of vertebrate hosts, causing diarrhea! diseases. Cattle are considered potential reservoirs of Cryptosporidium infection in humans, although their role in the transmission of E. bieneusi is not clear. In the present work, 793 fecal specimens from dairy cattle, native beef cattle, and water buffaloes on 11 farms in China were examined for the presence of Cryptosporidium spp. and E. bieneusi using nested PCR targeting the small subunit (SSU) rRNA gene of Cryptosporidium spp. and the internal transcribed spacer (ITS) of E. bieneusi. For Cryptosporidium, 144/446(32.3%) dairy cattle, 44/166(26.5%) beef cattle, and 43/181 (23.8%) water buffaloes were PCR-positive. Sequence analysis was successful for 213 of the 231 Cryptosporidium-positive isolates: among them 94 had Cryptosporidium andersoni, 61 had Cryptosporidium bovis, 54 had Cryptosporidium ryanae, 2 had a Cryptosporidium suis-like genotype, and 2 had mixed infections of C. bovis and C. ryanae. In dairy and beef cattle, C. andersoni and C. bovis were the most common species, whereas C. ryanae was the dominant species in water buffaloes. The latter species produced SSU rRNA sequences different between cattle and water buffaloes. For E. bieneusi, the infection rate of E. bieneusi in dairy cattle, beef cattle and water buffaloes was 4.9%, 5.4% and 2.2%, respectively. All 35 E. bieneusi-positive specimens were successfully sequenced, revealing the presence of four genotypes: three Group 2 genotypes previously reported in cattle as well as humans (I, J and BEB4) and one Group 1 genotype recently reported in yaks (CHN11). Genotypes I and J were the most common genotypes in dairy and beef cattle, while genotype CHN11 was the only genotype seen in water buffaloes. Thus, the distribution of Cryptosporidium spp. and E. bieneusi in water buffaloes might be different from in dairy and beef cattle in China. These findings indicate that some of the Cryptosporidium species and all four E. bieneusi genotypes identified in bovine animals in the study areas may have zoonotic potential. (C) 2014 Elsevier B.V. All rights reserved.