文献类型：期刊文献

英文题名：Molecular Detection of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria in High-Temperature Petroleum Reservoirs

作者：Li, Hui[1,2];Chen, Shuo[3,4];Mu, Bo-Zhong[3,4];Gu, Ji-Dong[1,5]

机构：[1]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China;[2]E China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Biorcactor Engn, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[5]Univ Hong Kong, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China

年份：2010

卷号：60

期号：4

起止页码：771

外文期刊名：MICROBIAL ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000284255700008)】；

基金：This study was supported by Environmental Toxicology Education Fund (to J-D G), National Natural Science Foundation of China (50744016), National High Technology Research and Development Program of China (2009AA063503), China Postdoctoral Science Foundation funded project (20080440082, 200902223), Shanghai Municipal Science and Technology Commission (071607014), and Shanghai Postdoctoral Scientific Program (09R21411900). We thank Jessie Lai for laboratory assistance and Meng Li for useful discussion.

语种：英文

摘要：Anaerobic ammonium-oxidizing (anammox) process plays an important role in the nitrogen cycle of the worldwide anoxic and mesophilic habitats. Recently, the existence and activity of anammox bacteria have been detected in some thermophilic environments, but their existence in the geothermal subterranean oil reservoirs is still not reported. This study investigated the abundance, distribution and functional diversity of anammox bacteria in nine out of 17 high-temperature oil reservoirs by molecular ecology analysis. High concentration (5.31-39.2 mg l(-1)) of ammonium was detected in the production water from these oilfields with temperatures between 55 degrees C and 75 degrees C. Both 16S rRNA and hzo molecular biornarkers indicated the occurrence of anammox bacteria in nine out of 17 samples. Most of 16S rRNA gene phylotypes are closely related to the known anammox bacterial genera Candidatus Brocadia, Candidatus Kuenenia, Candidatus Scalindua, and Candidatus Jettenia, while hzo gene phylotypes are closely related to the genera Candidatus Anammoxoglobus, Candidatus Kuenenia, Candidatus Scalindua, and Candidatus Jettenia. The total bacterial and anammox bacterial densities were 6.4 +/- 0.5x10(3) to 2.0 +/- 0.18x10(6) cells ml(-1) and 6.6 +/- 0.51x10(2) to 4.9 +/- 0.36x10(4) cell ml(-1), respectively. The cluster I of 16S rRNA gene sequences showed distant identity (<92%) to the known Candidatus Scalindua species, inferring this cluster of anammox bacteria to be a new species, and a tentative name Candidatus "Scalindua sinooil-field" was proposed. The results extended the existence of anammox bacteria to the high-temperature oil reservoirs.