文献类型：期刊文献

英文题名：A novel synthetic fluoro-containing jasmonate derivative acts as a chemical inducing signal for plant secondary metabolism

作者：Qian, Zhi-Gang[1]; Zhao, Zhen-Jiang[2]; Xu, Yufang[2]; Qian, Xuhong[2]; Zhong, Jian-Jiang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Pesticides & Pharmaceut, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2005

卷号：68

期号：1

起止页码：98

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：2005369339807);WOS:【SCI-EXPANDED(收录号:WOS:000230859900015)】；

语种：英文

外文关键词：Ammonia - Biosynthesis - Cells - Enzymes - Metabolites - Plants (botany)

摘要：A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plant cell system. The presence of novel synthesized pentafluoropropyl jasmonate (PFPJA) induced two early and important events in plant defense responses, including an oxidative burst and activation Of L-phenylalanine ammonia lyase. In addition, PFPJA was found to significantly increase Tc accumulation, without any inhibition of cell growth. Moreover, Tc accumulation was increased more in the presence of PFPJA compared with methyl jasmonate (MJA) and previously reported trifluoroethyl jasmonate (TFEJA). For example, addition of 100 mu M PFPJA on day 7 led to a high Tc content (38.2 +/- 0.3 mg/g) at day 2 1, while the Tc content was 29.3 +/- 0.3 mg/g and 34.9 +/- 0.9 mg/g with the addition of 100 mu M MJA and TFEJA, respectively. Quantitative structure-activity analysis of fluoro-containing jasmonates suggests that the increase in the fluoro-groups introduced into the carboxyl side-chain of MJA resulted in a higher stimulatory activity for Tc biosynthesis, which corresponds well with the markedly increased lipophilicity after fluorine introduction. These results indicate that newly synthesized fluoro-containing PFPJA can act as a powerful chemical inducing signal for secondary metabolism in plant cell cultures.