文献类型：期刊文献

英文题名：Direct Reductive Amination of Biobased Furans to N-Substituted Furfurylamines by Engineered Reductive Aminase

作者：Yang, Zi-Yue[1];Hao, Ya-Cheng[1];Hu, Song-Qing[1];Zong, Min-Hua[1];Chen, Qi[2];Li, Ning[1]

机构：[1]South China Univ Technol, Sch Food Sci & Engn, 381 Wushan Rd, Guangzhou 510640, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：363

期号：4

起止页码：1033

外文期刊名：ADVANCED SYNTHESIS & CATALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000605411400001)】；

基金：This research was financially supported by the National Natural Science Foundation of China (21676103 and 31971380), and the Guangzhou Municipal Science and Technology Project (201804010179 and 201803010080).

语种：英文

外文关键词：amines; biobased furans; enzyme catalysis; reductive aminases; site-directed mutagenesis

摘要：Furfurylamines are important building blocks for the synthesis of many pharmacologically active compounds and polymers. In this work, direct reductive amination of biobased furans to N-substituted furfurylamines by reductive aminase from Aspergillus oryzae (AspRedAm) was reported. Besides the reductive aminase activity, AspRedAm also showed a promiscuous, yet low alcohol dehydrogenase activity. The variant W210F proved to be a good catalyst for the synthesis of N-substituted furfurylamines. Furans were transformed to the target products with the conversions up to >99% and selectivities up to >99%. In addition, N-substituted furfurylamines were synthesized in the total turnover number (TTN) up to 3200 on a preparative scale, indicating the applicability of this biocatalytic route in synthetic chemistry.