文献类型：期刊文献

英文题名：A novel method combining aptamer-Ag10NPs based microfluidic biochip with bright field imaging for detection of KPC-2-expressing bacteria

作者：Chen, Jing[1];Li, Hui[1];Xie, Hexin[2];Xu, Danke[1]

机构：[1]Nanjing Univ, Sch Chem Engn, State Key Lab Analyt Chem Life Sci, 163 Xianlin Ave, Nanjing 210023, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：1132

起止页码：20

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20203309042764);WOS:【SCI-EXPANDED(收录号:WOS:000579365700003)】；

基金：We acknowledge financial support of the National Natural Science Foundation of China (21775068, 21475060, 21227009, and 21405077) and Excellent Research Program of Nanjing University (ZYJH004).

语种：英文

外文关键词：Bacteria; Microfluidic chip; Bright field; Aptamer; Nanoparticle

摘要：The beta-lactam drugs resistance poses a serious threat to human health throughout the world. Klebsiella pneumoniae carbapenemase 2 (KPC-2) is a carbapenemase that produced in bacteria can hydrolyze carbapenems, which typically considered as the antibiotics of last resort. Therefore, there is an urgent need to quickly and accurately detect whether bacteria express KPC-2. In this paper, a PDMS/glass microfluidic biochip integrated with aptamer-modified Ag(10)NPs nano-biosensors was developed for rapid, simple and specific pathogenic bacteria detection, more importantly, the biochip was combined with bright field imaging, then the captured bacteria could be observed and counted directly without using extra chemical labeling. KPC-2-expressing Escherichia coli (KPC-2 E.coli) was used as the target bacterium with a detected limit of 10(2) CFU and capture efficiency exceeded 90%. This method is remarkably specific towards KPC-2 E.coli over other non-resistant bacteria, and pathogen assay only takes similar to 1 h to complete in a ready-to-use microfluidic biochip. Furthermore, the effective capture and fast counting of microfluidic biochip system demonstrates its potential for the rapid detection of antibiotic-resistant bacteria. (C) 2020 Elsevier B.V. All rights reserved.