文献类型：期刊文献

英文题名：A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors

作者：Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：29

期号：3

起止页码：431

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000244293000014)】；

语种：英文

外文关键词：plasmid vector pEC; compatible origin; copy number; stability; dual plasmids expression; gene regulation

摘要：A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an L-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system.