文献类型：期刊文献

英文题名：Constructing a FRET-based molecular chemodosimeter for cysteine over homocysteine and glutathione by naphthalimide and phenazine derivatives

作者：Yang, Lin;Qu, Weisong;Zhang, Xiao;Hang, Yandi;Hua, Jianli[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：140

期号：1

起止页码：182

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000345824700020)】；

基金：This work was supported by the National Basic Research 973 Program (2013CB733700), NSFC/China (21372082, 2116110444, 91233207 and 21172073), the Science Fund for Creative Research Groups (21421004). J.-L. Hua very much appreciates Prof. H. Tian's helpful discussions and valuable comments.

语种：英文

摘要：A molecular chemodosimeter PHSN, with a naphthalimide fluorophore connected to a newly designed phenazine energy acceptor, for the selective detection of cysteine was effectively synthesized. PHSN featured efficient intramolecular fluorescence resonance energy transfer (FRET) based on spectral overlap between the emission of naphthalimide and the absorption of phenazine. A cystamine chain with a S-S bond was applied to play the role of recognition moiety and the linker part. The specific reaction between the biological thiols and PHSN gave rise to an obvious fluorescence intensity enhancement at 540 nm, which is induced by cleavage of the disulfide bond followed by elimination of the FRET effect. High sensitivity and selectivity for cysteine over homocysteine and glutathione were also achieved. In addition, upon excitation at 400 nm, a relatively weak NIR emission provided an internal standard making PHSN a promising ratiometric sensor for cellular detection of cysteine.