文献类型：期刊文献

英文题名：In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor

作者：Jiang, Lei[1];He, Cai-Hong[1];Chen, Hua-Ying[1];Xi, Cheng-Ye[1];Fodjo, Essy Kouadio[1,2];Zhou, Ze-Rui[1];Qian, Ruo-Can[1];Li, Da-Wei[1];Hafez, Mahmoud Elsayed[1,3]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Felix Houphouet Boigny Univ, Lab Phys Chem, Abidjan 225, Cote Ivoire;[3]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2021

卷号：93

期号：37

起止页码：12609

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20213910957297);WOS:【SCI-EXPANDED(收录号:WOS:000699948200014)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 21788102, 21974046, 21777041, 22176058, and 21977031), Science and Technology Commission of Shanghai Municipality (Grant No. 19391901700, 19520744000, and 19ZR1472300), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Catalyst activity - Organometallics - Oxidation - Metal nanoparticles - Raman scattering - Disease control - Gold nanoparticles - Diagnosis - Surface scattering

摘要：Hydrogen peroxide (H2O2) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H2O2 assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H2O2 released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H2O2-responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H2O2 by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H2O2 detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H2O2 detection. Furthermore, the developed SERS sensor enables real-time detection of H2O2 secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal-organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H2O2 secretion, thus exhibiting promising potential for future therapies.