文献类型：期刊文献

英文题名：Identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry

作者：Zhang, Hongyang[2];Yang, Huihua[1];Zhang, Min[3];Wang, Yuerong[2];Wang, Jingrong[4];Yau, Leefong[4];Jiang, Zhihong[4];Hu, Ping[2]

机构：[1]Guilin Univ Elect Technol, Coll Comp Sci & Control, Guilin 541004, Peoples R China;[2]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China;[4]Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macao, Peoples R China

年份：2012

卷号：25

期号：2

起止页码：142

外文期刊名：JOURNAL OF FOOD COMPOSITION AND ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000302423200005)】；

基金：This study was financially supported by the National Natural Science Foundation of China (Nos. 30860381 and 21006023). We also express our gratitude to Dr. Lee Kim-Chung (Agilent Technologies Hong Kong Ltd.), and to Mr. Chan Chi-Leung (Hong Kong Baptist University) for technical support in UHPLC/QTOF-MS analysis.

语种：英文

外文关键词：Luo-Han-Guo; Siraitia grosvenorii; Food analysis; Food composition; Flavonol and triterpene glycosides; Ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry; Liquid chromatography-mass spectrometry LC-MS; Growing periods; Principle component analysis

摘要：In this study, a method for the identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) with personal database matching has been developed. Two flavonol glycosides and 20 triterpene glycosides were identified, and in addition the profiles of Luo-Han-Guo samples collected at different growing periods (15-, 40-, and 80-days) were compared. Principle component analysis (PCA) showed a clear separation among the various samples from different growing periods. The content of mogroside V significantly increased, while the level of mogroside IIE class dramatically decreased with an increase of growing time: however, the content of mogroside IV class was found to be high after a 40-day period of accumulation, but it subsequently decreased in the fully ripe period. Finally, 7 mogrosides could be screened as potential markers for discrimination of Luo-Han-Guo samples at different stages. The proposed analytical method combined with multivariate statistical analysis was shown to be a useful tool for investigating the chemical components of Luo-Han-Guo as well as for its quality evaluation. (C) 2011 Elsevier Inc. All rights reserved.