文献类型：期刊文献

英文题名：Identification of Potential Binding Sites of Sialic Acids on the RBD Domain of SARS-CoV-2 Spike Protein

作者：Li, Bingqian[1,2,3];Wang, Lin[1,2];Ge, Huan[4];Zhang, Xianglei[1,2];Ren, Penxuan[1,2];Guo, Yu[5,6];Chen, Wuyan[7];Li, Jie[7];Zhu, Wei[1,2];Chen, Wenzhang[1,2];Zhu, Lili[4];Bai, Fang[1,2]

机构：[1]ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China;[2]ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China;[3]Imperial Coll London, Dept Chem, London, England;[4]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai, Peoples R China;[5]Nankai Univ, State Key Lab Med Chem Biol, Tianjin, Peoples R China;[6]Nankai Univ, Coll Pharm, Tianjin, Peoples R China;[7]Natl Ctr Prot Sci Shanghai, Shanghai, Peoples R China

年份：2021

卷号：9

外文期刊名：FRONTIERS IN CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000687986900001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality grants (Grant IDs: 20431900102, 20431900100, 2043190012, and 20430780300); the startup package from ShanghaiTech University; the National Natural Science Foundation of China (NSFC)(Grant ID: 82003654t) and the National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China (Grant ID: 2018ZX09711002); and Shanghai Science and Technology Development Funds (Grant ID: 20QA1406400).

语种：英文

外文关键词：SARS-CoV-2; receptor; sialic acid; RBD domain; spike protein

摘要：COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still an emergent pandemic for humans. The virus infection is achieved by penetrating its spike protein to host cells via binding with ACE2. Moreover, recent studies show that SARS-CoV-2 may have multiple receptors that need to be further revealed. SARS-CoV-2 shares similar sequences of the spike protein with the Middle East Respiratory Syndrome Coronavirus (MERS-CoV), which can invade host cells by binding to either DPP4 or sialic acids. Sialic acids can be linked to the terminal of glycoproteins and gangliosides are used as one of the receptors of many types of viruses. Therefore, it is very interesting to determine whether sialic acid is a potential receptor of SARS-CoV-2. To address this question, we took N-Acetylneuraminic acid (Neu5Ac), a type of predominant sialic acid found in human cells, as the molecular probe to computationally search the surface of the spike protein to locate the potential binding sites of Neu5Ac. SPR analysis and mass spectrum analysis confirmed the interaction between Neu5Ac and spike protein. This study shows that sialic acids can moderately interact with the spike protein of SARS-CoV-2 by binding between the two RBDs of the spike protein, indicating it could be a potential secondary or auxiliary receptor of SARS-CoV-2.