文献类型：期刊文献

英文题名：In-depth exploration of spermidines in Goji berry: the identification and semi-quantitation of spermidines by liquid chromatography-tandem mass spectrometry with feature-based molecular network and high performance liquid chromatography

作者：Chen, Shiyi[1];Sang, Bin[1];Tao, Xinyu[1];Miao, Zhenhua[2];Ling, Meng[1];Jia, Qiangqiang[3];Zhang, Min[4];Wang, Yuerong[1];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Ningxia Med Univ, Sch Basic Med Sci, Dept Anat, Yinchuan 750004, Peoples R China;[3]Qinghai Univ, Coll Pharm, Xining 810016, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2026

卷号：517

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20261920662478);WOS:【SCI-EXPANDED(收录号:WOS:001765515100001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant number 81973285) . And thanks to Agilent for their instrumental support of this work.

语种：英文

外文关键词：Goji berry; HPLC; LC-MS; FBMN; Spermidine

摘要：In this study, 164 compounds, including 43 spermidines and 5 spermines, were identified from Lycium barbarum (Zhongning, China) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with featurebased molecular network (FBMN). The high performance liquid chromatography (HPLC) fingerprint of Goji berry was established, and 16 common peaks were assigned, 13 of which corresponded to spermidines derivatives. Fingerprint similarities of 23 batches ranged from 0.742 to 0.933 relative to the reference chromatogram. Using the self-purified N1-N10-dihydrocaffeoyl-caffeoyl-spermidine-di-glucoside as reference standard, an HPLC quantitative method for 1 spermidine and semi-quantitative method for 15 spermidines in Goji berry was developed and validated (linear range: 4.68-574.53 mu g/mL, correlation coefficient (R2): 0.9990, recovery rate: 96.4%-101.3%, relative standard deviation (RSD): 1.57%). The total content of 16 spermidines across 23 batches ranged from 2.08 to 4.98 mg/g. These methods are applicable to the quality assessment, offering an analytical framework for the characterization of spermidine type constituents in Goji berry.