文献类型：期刊文献

英文题名：Biosynthesis of 1,4-butanediol from erythritol using whole-cell catalysis

作者：Dai, Lu[1,2];Tai, Cui[2];Shen, Yaling[1];Guo, Yali[1,2];Tao, Fei[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

年份：2019

卷号：37

期号：2

起止页码：92

外文期刊名：BIOCATALYSIS AND BIOTRANSFORMATION

收录：;EI(收录号：20181705049927);WOS:【SCI-EXPANDED(收录号:WOS:000460391700003)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality [17JC1404800], and the grants from the National Natural Science Foundation of China [31570101].

语种：英文

外文关键词：1,4-Butanediol; erythritol; whole-cell biocatalysis

摘要：1,4-Butanediol (BDO) biosynthesis from renewable resources is of increasing interest because of global energy and environmental problems. We have previously demonstrated the production of BDO from erythritol by whole-cell catalysis. Here, the effects of several variables on BDO production were investigated, including cell density, temperature, substrate concentration and pH. It was found that the maximum BDO production was obtained at cell density (OD600) of 30. Low temperature and weak alkaline environment were beneficial for the biotransformation. Regarding substrate concentration, 80 g/L of erythritol was found to be optimum for the bioconversion. Under the optimal conditions, the highest concentration of BDO reached 34.5 mg/L, resulting in 5.8-fold increment after optimization. These results will provide useful guidance for enhancing the bioconversion of erythritol to BDO.