文献类型：期刊文献

英文题名：Fine-Tuning Biosynthesis of Biodegradable Poly(3-hydroxybutyrate-co-lactate) with Different Ratios of D-Lactate from Xylose by Engineered Escherichia coli

作者：Wu, Ju[1];Gong, Xuan[1];Sun, Xinye[1];Guo, Pengye[1];Luo, Yuanchan[1];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Me, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China;[3]Natl Light Ind Council, Key Lab Biobased Mat Engn China, Shanghai 200237, Peoples R China

年份：2023

卷号：11

期号：41

起止页码：15043

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20234615049810);WOS:【SCI-EXPANDED(收录号:WOS:001077686500001)】；

基金：This work was supported by the National Natural Science Foundation of China (22278137), the National Key R&D Program of China (2021YFC2103500), and the Science and Technology Commission of Shanghai Municipality (21DZ1209100). The work was also supported by the Open Funding Project of the CAS-Key Laboratory of Synthetic Biology.

语种：英文

外文关键词：bioplastics; LA fraction; lePGT-1; cytochromeoxidase genes; bioreactor study

摘要：The toughness and transparency of poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] depend on its lactate (LA) fraction. In Escherichia coli, LA production can be increased by restricting the activity of the electron transport chain (ETC). As ubiquinone-8 (Q8) is essential to mediate electron transfer, it is one target to modulate the ETC in E. coli. 4-Hydroxybenzoate geranyltransferase (lePGT-1, encoded by the lepgt gene) from Lithospermum erythrorhizon can competitively consume substrates required for the biosynthesis of Q8. Therefore, flexible regulation of LA production can be achieved by controlling the expression level of the lepgt gene to precisely control the flux of the ETC. This strategy was applied for the regulation of the molar percentage (mol %) of the LA fraction in P(3HB-co-LA). Using xylose as a carbon source instead of glucose increased the LA fraction to 10.6-27.7 mol %, and deleting cytochrome oxidase genes further increased the LA fraction to 29.3-41.8 mol %. Finally, the stability and effectiveness of the flexible control strategy were verified by a bioreactor study.