文献类型：期刊文献

中文题名：大肠杆菌DH5α及其耐乙酸突变株DA19在碳源限制培养基中乙酸的积累

英文题名：Acetic Acid Accumulation in Escherichia coli DH5α and Its Acetate-Tolerant Mutant DA19 Cultured in Carbon-Limited Media

作者：张晓云[1];张艳军[1];李志敏[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2007

卷号：33

期号：4

起止页码：475

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20073810818844);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家863项目资助(2002AA217021)

语种：中文

中文关键词：大肠杆菌;突变株;培养基;乙酸;耐受性

外文关键词：Escherichia coli; mutant~ medium; acetic acid; tolerance

摘要：在碳源限制的基本培养基和复合培养基中分别对大肠杆菌DH5α及其耐乙酸突变株DA19进行分批培养,两者在复合培养基中的乙酸产量均明显高于基本培养基,相同培养基中DH5α的乙酸产量高于DA19。基本培养基中分批培养的物料衡算显示:DH5α通过三羧酸(TCA)循环和呼吸链提供能量的葡萄糖低于DA19,通过乙酸形成途径的量则增加,表明DA19的TCA循环或电子传递链氧化能力有所提高,而DH5α的TCA循环或电子传递链的能力限制使更多的葡萄糖通过形成乙酸来提供能量。能量需求的计算结果表明:在碳源限制的复合培养基中,有机氮源的异化代谢提供了部分能量,在丙氨酸和谷氨酸为碳氮源的基本培养基中的分批培养也表明氨基酸的异化代谢对乙酸产生的影响。
Batch cultivations of Escherichia coli DH5a and its acetate-tolerant mutant DA19 were performed in carbon-limited defined and complex media. The acetic acid production in the complex media was more than that in the defined medium. A decrease in acetic acid production in DA19 was observed compared with DH5α when cultured in the same medium. The carbon balance of batch cultivations in the defined medium showed that glucose degraded through tricarboxylic acid （TCA） cycle and respiration chain in DH5α was much less than that in DA19, and thus glucose catabolized through acetic acid production pathway was much more than that in DA19 to supply energy. This suggested that a lower capacity of TCA cycle and/or respiration chain of DH5α was responsible for more acetic acid production. The energy balance suggested that a part of the organic nitrogen sources was degraded to provide energy in addition to glucose when DH5α and DA19 were cultivated in carbon-limited complex media. Batch cultivations of DH5α and DA19 in a defined medium with alanine and glutamate as the carbon and nitrogen sources also indicated that the catabolism of amino acids contributed to acetic acid production.