文献类型：期刊文献

英文题名：Effects of divalent metal cations on lovastatin biosynthesis from Aspergillus terreus in chemically defined medium

作者：Jia, Zhihua[1]; Zhang, Xiaoli[2]; Zhao, Yaling[2]; Cao, Xuejun[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China; [2] College of Chemical Engineering, Northwest University, 710069 Xi'an, China

年份：2009

卷号：25

期号：7

起止页码：1235

外文期刊名：World Journal of Microbiology and Biotechnology

收录：EI(收录号：20092712161736)

语种：英文

外文关键词：Aspergillus - Metal ions - Metals - Growth kinetics - Biosynthesis - Positive ions - Biochemistry

摘要：Effects of six divalent metal cations: Fe2+, Ca2+, Zn2+, Mg2+, Cu2+and Mn2+ on fungal cell growth and lovastatin biosynthesis were investigated by submerged cultivation of Aspergillus terreus in a modified chemically defined medium. The influences of different initial concentrations of the above six metal cations were also examined at 1, 2, and 5 mM, respectively. Cu2+ apparently inhibited the cell growth, but had no influence on biosynthesis of lovastatin. All of Fe2+, Ca2+, Zn2+, Mg2+ and Mn2+ promoted the cell growth and lovastatin biosynthesis in different extents. The highest biomass of 13.8 ± 0.5 g l-1 and specific lovastatin titres of 49.2 ± 1.4 mg gDCW-1 were obtained at the level of 2 and 5 mM in the presence of Zn2+, respectively. The values were improved double and 14.4-fold. Excess Zn 2+ inhibited the cell growth, but enhanced lovastatin biosynthesis with an increment of 17.6 mg l-1 per mM. The interactions of all metal cations slightly inhibited the lovastatin production comparing with the existence of Zn2+, Fe2+ and Mg2+ solely, yet remarkably improved the cell growth. These results suggest that the divalent metal ions Zn2+ or Fe2+ influence the production by regulating the action of key enzymes such as LovD or LovF in lovastatin biosynthesis. ? 2009 Springer Science+Business Media B.V.