文献类型：期刊文献

英文题名：Hydroxylases involved in terpenoid biosynthesis: a review

作者：Zhang, Zihan[1];Wu, Qing-Yang[1];Ge, Yue[1];Huang, Zheng-Yu[1];Hong, Ran[2];Li, Aitao[3];Xu, Jian-He[1];Yu, Hui-Lei[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Organ Chem, CAS Key Lab Synthet Chem Nat Subst, Shanghai 200032, Peoples R China;[3]Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

年份：2023

卷号：10

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20233014427053);WOS:【SCI-EXPANDED(收录号:WOS:001029958900001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 21922804 and 32271540), the National Key Research and Development Program of China (Grants 2019YFA0905000 and 2021YFC2102900), Program of Shanghai Academic Research Leader (21XD1400800), and the Fundamental Research Funds for the Central Universities (Grant No. 22221818014).

语种：英文

外文关键词：Biooxidation; Cytochrome P450 monooxygenase; Hydroxylase; Natural products; Terpenoids

摘要：Terpenoids are pervasive in nature and display an immense structural diversity. As the largest category of plant secondary metabolites, terpenoids have important socioeconomic value in the fields of pharmaceuticals, spices, and food manufacturing. The biosynthesis of terpenoid skeletons has made great progress, but the subsequent modifications of the terpenoid framework are poorly understood, especially for the functionalization of inert carbon skeleton usually catalyzed by hydroxylases. Hydroxylase is a class of enzymes that plays an important role in the modification of terpenoid backbone. This review article outlines the research progress in the identification, molecular modification, and functional expression of this class of enzymes in the past decade, which are profitable for the discovery, engineering, and application of more hydroxylases involved in the plant secondary metabolism.