文献类型：期刊文献

英文题名：A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals

作者：Zhao, Zhangting[1];Ma, Xingyuan[1];Zhang, Ruihuan[1];Hu, Fabiao[1];Zhang, Tong[2];Liu, Yuping[2];Han, Myong Hun[1,3];You, Fang[4];Yang, Yi[5];Zheng, Wenyun[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai, Peoples R China;[3]KIM IL SUNG Univ, Fac Life Sci, Dept Genet, Pyongyang, North Korea;[4]Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore;[5]SinGENE Biotech Pte Ltd, Singapore Sci Pk, Singapore 118258, Singapore

年份：2021

卷号：35

外文期刊名：NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE

收录：;EI(收录号：20210109730967);WOS:【SCI-EXPANDED(收录号:WOS:000664181600006)】；

基金：This work was supported by the National Key Research and Development Project of China (2018YFA0902804) , the National Natural Science Foundation (31670944, 81673345) , and the Science and Technology Innovation Action Plan of Shanghai (17431904600) .

语种：英文

外文关键词：DNA vaccine; Liposome-polymer hybrid nanoparticles; Delivery; Immune evaluation; Infectious diseases

摘要：DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-MEG/LNPs) consisting of a biodegradable core (mPEG-PLGA) and a hydrophilic shell (lecithin/PEG-DSPE-Mal 2000) for delivering a multi-epitope self-replication DNA vaccine (pSFV-MEG). The pSFV-MEG/LNPs with optimal particle size (161.61 +/- 15.63 nm) and high encapsulation efficiency (87.60 +/- 8.73%) induced a strong humoral (3.22-fold) and cellular immune responses (1.60-fold) compared to PBS. Besides, the humoral and cellular immune responses of pSFV-MEG/LNPs were 1.58-and 1.05-fold than that of pSFV-MEG. All results confirmed that LNPs was a very promising tool to enhance the humoral and cellular immune responses of pSFV-MEG. In addition, the rational design and delivery platform can be used for the development of DNA vaccines for other infectious diseases. (c) 2020 Published by Elsevier Inc.