文献类型：期刊文献

英文题名：Highly Efficient Folding of Multi-Disulfide Proteins in Superoxidizing Escherichia coli Cytoplasm

作者：Zhang, Wenyao[1];Zheng, Wenyun[1];Mao, Miaowei[1];Yang, Yi[1]

机构：[1]E China Univ Sci & Technol, Synthet Biol & Biotechnol Lab, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn,Sch Pharm, Shanghai 200237, Peoples R China

年份：2014

卷号：111

期号：12

起止页码：2520

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20143600010879);WOS:【SCI-EXPANDED(收录号:WOS:000344388600016)】；

基金：Contract grant sponsor: 863 Program Contract grant number: 2006AA02Z160 Contract grant sponsor: NSFC Grant numbers: 31225008; 91313301; 31071260; 31170815 Contract grant sponsor: The Fok Ying Tung Education Foundation Contract grant number: 111022 Contract grant sponsor: The Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institution of Higher Learning, the Dawn Program of Shanghai Education Commission Contract grant number: 11SG31 Contract grant sponsor: The 111 Project Contract grant number: B07023

语种：英文

外文关键词：redox potential; disulfide formation; oxidative protein folding; superoxidizing cells

摘要：In this study, we monitored the thiol-disulfide redox potential of different Escherichia coli strains using redox-sensitive variants of green fluorescent protein. The cells with extreme oxidizing cytoplasm were generated by introducing a highly efficient disulfide relay system. The developed cells have exceptionally efficient de novo disulfide bond formation and significantly improve the oxidative folding of the client multi-disulfide proteins. Superoxidizing E. coli strain provides an effective method for the high-level production of recombinant disulfide-containing proteins. (C) 2014 Wiley Periodicals, Inc.